고농도의 포도당이 처리된 MC3T3-E1 세포에서 635 ㎚ 광조사에 의한 골분화에 관한 연구
Regulation of Osteoblast Differentiation by 635 ㎚ Irradiation in MC3T3-E1 Cells Supplemented with High Glucose
임원봉, 원재웅, 김지선, 김인애, 고영종, 권혁일, 김상우, 김서연, 김옥준, 최홍란,
소속 상세정보
임원봉 ( Lim Won-Bong ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
원재웅 ( Won Jae-Woong ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김지선 ( Kim Ji-Sun ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김인애 ( Kim In-Ae ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
고영종 ( Ko Young-Jong ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
권혁일 ( Kwon Hyuk-Il ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김상우 ( Kim Sang-Woo ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김서연 ( Kim Seo-Yune ) - 송원대학 치위생과
김옥준 ( Kim Ok-Joon ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
최홍란 ( Choi Hong-Ran ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
KMID : 0829220110350010019
Abstract
Diabetic patients tend to exhibit delayed bone formation and osteoblast differentiation, which results in osteopenia. Recently, numerous clinical reports suggest that 635-㎚ light irradiation improves bone regeneration and wound healing, and reduces pain in patients suffering from diabetes. The purpose of the present study was to test the hypothesis that 635-㎚ irradiation can influence bone formation by MC3T3-E1 osteoblasts cultured on high concentrations of glucose(25mmol/L D-glucose) in the presence or absence of phorbol 12-myristate 13-acetate(PMA), and to establish an in vitro pathological model of bone formation. The effect of 635-㎚ irradiation on bone formation was investigated using Alizarin Red S staining, and alkaline phosphatase enzyme activ ity and calcium deposition assays. In addition, gene expression of the o steogenic markers BMP-2, osterix and osteocalcin were assayed by RT-PCR. Calcium deposition by MC3T3-E1 cells was reduced in the presence of high concentrations of glucose or by PMA supplementation. However, 635-㎚ irradiation led to an increase in calcium deposition by MC3T3 cells, followed by increased bone mineralization. mRNA expression of BMP-2 and osterix at an early stage and of osteocalcin at a late stage was significantly upregulated by 635-㎚ irradiation in MC3T3-E1 cells supplemented with high concentrations of glucose. Irradiation at 635 ㎚ increases bone mineralization in MC3T3-E1 cells cultured in vitro on high concentrations of glucose and alters osteogenic gene expression, which accelerates bone formation in hyperglycemic conditions.
키워드
Diabetes; Bone; 635-㎚ irradiation
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